Confocal Microscope

Description: Olympus Microscope with CARVII spinning disc confocal system
Location: Chapman Hall - 225
Uses:

This microscope can do a number of different experiments including confocal, widefield fluorescence, brightfield, DIC and phase imaging.

Specifications:

Objective - Numerical Aperture
10x - 0.3
20x - 0.45
40x - 0.6
60x, water - 1.2
100x, oil - 1.3
100x, oil - 1.4

Filter Set - Excitation - Emission
DAPI - 381-393nm - 417-477nm
GFP - 464-500nm - 464-500nm
TxRED - 542-582nm - 604-644nm

Camera - Resolution - Sensitivity - Max Frame Rate
Hamamatsu ORCA - High, 1344 x 1024 - Medium - ~5 full frames/sec
Photometrics QuantEM - Medium, 512 x 512 - High - ~30 full frames/sec

Download Operating Procedure